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Journal / Biology / Su larga scala Knockdown Gene in
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JoVE Journal Biology
Su larga scala Knockdown Gene in
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Su larga scala Knockdown Gene in

Article DOI: 10.3791/50693-v • 18:38 min • September 25th, 2013
September 25th, 2013 •
1, 2, 3
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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Mentre dsRNA alimentazione in C. elegans è un potente strumento per valutare la funzione del gene, i protocolli attuali schermi di alimentazione su larga scala producono efficienze atterramento variabili. Descriviamo un protocollo migliorato per eseguire larga scala RNAi schermi alimentazione che si traduce in knockdown altamente efficace e riproducibile di espressione genica.

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Biologia dello Sviluppo Numero 79 Caenorhabditis elegans (C. elegans) Tecniche di Gene Knockdown, Interferenze dsRNA gene knockdown schermo alimentazione su larga scala
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