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Immunology and Infection

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JoVE Journal Immunology and Infection
De células vivas de Alphaherpes Vírus Transporte anterógrada e Spread
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A Non-invasive Way to Isolate and Phenotype Cells from the Conjunctiva
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De células vivas de Alphaherpes Vírus Transporte anterógrada e Spread

Article DOI: 10.3791/50723-v • 15:31 min • August 16th, 2013
August 16th, 2013 •
1,2, 2, 2
1Department of Immunology and Infectious Diseases, Montana State University, 2Department of Molecular Biology, Princeton University

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Imagem de infecções por vírus alphaherpes células vivas permite a análise dos eventos dinâmicos de transporte e dirigido propagação intercelular. Apresentamos aqui as metodologias que utilizam estirpes de vírus recombinantes que expressam proteínas de fusão fluorescentes para facilitar a visualização dos conjuntos virais durante a infecção de neurónios primários.

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Virologia Infecção Imunologia Medicina Biologia Molecular Biologia Celular Microbiologia Genética Microscopia de fluorescência Neurobiologia vírus Herpes proteína fluorescente microscopia de epifluorescência cultura neuronal axonal virion microscopia de vídeo vírus células vivas imagem
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