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JoVE Journal Chemistry
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Article DOI: 10.3791/50726-v • 08:50 min • October 10th, 2013
October 10th, 2013 •
1, 1,2, 1, 3, 1
1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University

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We describe a procedure for profiling salivary proteins using multiplexed microsphere-based antibody arrays. Monoclonal antibodies were covalently linked to fluorescent dye-encoded 4.5 μm polymer microspheres using carbodiimide chemistry. The modified microspheres were deposited in fiber-optic microwells to measure protein levels in saliva using fluorescence sandwich immunoassays.

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Multiplexed Fluorescent Microarray Human Salivary Protein Analysis Polymer Microspheres Fiber-optic Bundles Immuno-array Technology Suspension Array Fabrication Fluorescence Microscopy Polymer Microspheres Fluorescent Dyes Capture Antibodies EDC/NHS Coupling Chemistry Protein Microarray Microwells Chemically Etched Fiber-optic Bundle Sandwich Immunoassay Biotinylated Detection Antibodies Streptavidin-conjugated Fluorescent Probe R-phycoerythrin
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