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JoVE Journal
Chemistry

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复用荧光微阵列人类唾液蛋白质分析用高分子微球和光纤束
 
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复用荧光微阵列人类唾液蛋白质分析用高分子微球和光纤束

Article DOI: 10.3791/50726-v 08:50 min October 10th, 2013
October 10th, 2013

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Summary

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Click here for the English version.

我们描述了使用多路复用基于微球的抗体阵列分析唾液蛋白的过程。单克隆抗体共价连接到荧光染料编码的4.5微米聚合物用碳二亚胺化学性微球。修改后的微球沉积在光纤的微孔用荧光夹心免疫测定蛋白水平在唾液。

Tags

化学,第80,蛋白检测,微阵列,复用荧光定量,光纤传感器,基于微球的免疫测定,唾液分析,微球编码
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