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Multiplex fluorescente Microarray per salivare umana Protein Analysis Utilizzando polimerici Microsfere e fasci di fibre ottiche
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Multiplex fluorescente Microarray per salivare umana Protein Analysis Utilizzando polimerici Microsfere e fasci di fibre ottiche

Article DOI: 10.3791/50726-v • 08:50 min • October 10th, 2013
October 10th, 2013 •
1, 1,2, 1, 3, 1
1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University

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Descriviamo una procedura per la profilatura proteine ​​salivari utilizzo di matrici di anticorpi a base di microsfere-multiplex. Gli anticorpi monoclonali sono stati legati covalentemente a colorante fluorescente con codifica 4.5 micron microsfere polimeriche utilizzando la chimica carbodiimmide. Le microsfere modificati sono stati depositati in pozzetti in fibra ottica per misurare i livelli di proteine ​​nella saliva mediante saggi immunologici panino fluorescenza.

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Chimica il rilevamento di proteine microarray quantificazione fluorescente multiplex biosensore in fibra ottica immunologici a base di microsfere l'analisi della saliva la codifica microsfere
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