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JoVE Journal Chemistry
Multiplexed Fluorescent Mikromatrise for Menneskelig Spytt Protein Analysis Bruke Polymer microspheres og Fiberoptisk Bundles
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Multiplexed Fluorescent Mikromatrise for Menneskelig Spytt Protein Analysis Bruke Polymer microspheres og Fiberoptisk Bundles

Article DOI: 10.3791/50726-v • 08:50 min • October 10th, 2013
October 10th, 2013 •
1, 1,2, 1, 3, 1
1Department of Chemistry, Tufts University, 2Department of Analytical Chemistry, Complutense University (Spain), 3Department of Electrical and Computer Engineering, Tufts University

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Vi beskriver en fremgangsmåte for profilering av spyttproteiner ved bruk av multipleksede mikrosfære-baserte antistoff-matriser. Monoklonale antistoffer ble kovalent bundet til fluorescerende fargestoff-kodede 4,5 um og polymer-mikrosfærer ved bruk av karbodiimid-kjemi. De modifiserte mikrokuler ble avsatt i fiberoptiske mikrobrønner for å måle proteinnivåer i spytt ved hjelp av fluorescens sandwich-immunoanalyser.

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Kjemi protein sensing microarray multiplekse fluorescerende kvantifisering fiberoptisk biosensor mikrokulebaserte immunoassays spytt analyse microsphere koding
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