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Immunology and Infection

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JoVE Journal Immunology and Infection
Total Protein Extraction och 2-D-gelelektrofores Metoder för
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Total Protein Extraction och 2-D-gelelektrofores Metoder för

Article DOI: 10.3791/50730-v • 08:31 min • October 15th, 2013
October 15th, 2013 •
1, 1, 1, 1
1Department of Pediatrics, Centre for Understanding and Preventing Infection in Children, University of British Columbia

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Medlemmar av Burkholderia släktet är patogener av klinisk betydelse. Vi beskriver ett förfarande för att totalt bakteriellt protein extraktion, med användning av mekanisk sönderdelning och 2-D-gelelektrofores för efterföljande proteomic analys.

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Immunologi bakterier Aerobic gramnegativa bakterier Immunsystemets sjukdomar Respiratory Tract Diseases, Proteiner glaspärlor 2-D-gelelektrofores
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