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Immunology and Infection

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JoVE Journal Immunology and Infection
Identifikation DNA-mutationer i renset hæmatopoietisk stamcelletransplantation / stamceller
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Identifikation DNA-mutationer i renset hæmatopoietisk stamcelletransplantation / stamceller

Article DOI: 10.3791/50752-v • 11:06 min • February 24th, 2014
February 24th, 2014 •
1, 1,2, 1, 3, 4,5, 2, 2,5, 1,2,5
1Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, 2Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, 3Department of Pathology, UT Health Science Center at San Antonio, 4Department of Microbiology, UT Health Science Center at San Antonio, 5Cancer Therapy and Research Center, UT Health Science Center at San Antonio

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Her beskriver vi en in vivo-mutagenese analyse for et lille antal af oprensede hæmatopoietiske celler ved anvendelse af LacI transgene musemodel. LacI genet kan isoleres til at bestemme frekvens, placering og type af DNA mutanter spontant opstået eller efter udsættelse for genotoxins.

Tags

Infektion , DNA-mutationer,
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