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Rivelare l'organizzazione del citoscheletro delle cellule tumorali invasive in 3D
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Rivelare l'organizzazione del citoscheletro delle cellule tumorali invasive in 3D

Article DOI: 10.3791/50763-v • 11:09 min • October 26th, 2013
October 26th, 2013 •
1, 1, 1
1UMR 144 CNRS, Institut Curie

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Questo articolo presenta un metodo per etichettare fluorescently collagene che può essere ulteriormente utilizzato sia per correzione e di imaging vivo di colture cellulari 3D. Forniamo anche un protocollo ottimizzato per visualizzare endogene proteine ​​del citoscheletro delle cellule coltivate in ambienti 3D.

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