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Avslöjar cytoskeletal organisationen av invasiva cancerceller i 3D

Article DOI: 10.3791/50763-v • 11:09 min • October 26th, 2013
October 26th, 2013 •
1, 1, 1
1UMR 144 CNRS, Institut Curie

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I denna artikel presenteras en metod för att fluorescerande märka kollagen som ytterligare kan användas för både fix och levande avbildning av 3D cellkulturer. Vi erbjuder också ett optimerat protokoll för att visualisera endogena cytoskelettproteiner av celler odlade i 3D-miljöer.

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Medicin 80 TAMRA kollagen 3D matrix spheroids F-aktin mikrotubuli
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