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同時電気生理学的記録および視交叉上核ニューロンのカルシウムイメージング
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

同時電気生理学的記録および視交叉上核ニューロンのカルシウムイメージング

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09:42 min

December 08, 2013

DOI:

09:42 min
December 08, 2013

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Summary

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Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

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