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Journal / Medicine / Isolation, Kultur og Imaging for Human Føtal…
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Isolation, Kultur og Imaging for Human Føtal pankreascelle Clusters
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Isolation, Kultur og Imaging for Human Føtal pankreascelle Clusters

Article DOI: 10.3791/50796-v • 08:09 min • May 18th, 2014
May 18th, 2014 •
1, 1, 1, 1
1Pediatric Diabetes Research Center, Department of Pediatrics, University of California, San Diego

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En protokol til at isolere, er kultur og image ø-celle klynger (ICCS) afledt af humane føtale bugspytkirtelceller beskrevet. Metoden beskriver de er nødvendige for at generere ICC fra væv, kultur som monolag eller i suspension som aggregater, og image for markører af spredning og pancreas celle skæbne beslutninger trin.

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Medicine humane føtale bugspytkirtel ø-celle klynge (ICC) transplantation immunfluorescens endokrine celleproliferation differentiering C-peptid
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