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JoVE Journal Biology
Skyv forberedelsesmetode for å bevare tredimensjonal kromatinarkitektur av testikkel bakterieceller
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Skyv forberedelsesmetode for å bevare tredimensjonal kromatinarkitektur av testikkel bakterieceller

Article DOI: 10.3791/50819-v • 07:34 min • January 10th, 2014
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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Materialet her beskriver en metode utviklet for å bevare den tredimensjonale kromatinstrukturen til testikulære bakterieceller. Dette kalles den tredimensjonale (3D) lysbildemetoden. Denne metoden forbedrer følsomheten for påvisning av subnukleære strukturer og gjelder for immunfluorescens, DNA og RNA fluorescens in situ hybridisering (FISH).

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Grunnleggende protokoll Utgave 83 Kromatin Bakterieceller Sexkromosomer Testis Meiotisk sexkromosominaktivering Postmeiotisk sexkromatin
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