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Slide Forberedelse Metode til at bevare tre-dimensionelle Chromatin Arkitektur testikelceller
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Slide Forberedelse Metode til at bevare tre-dimensionelle Chromatin Arkitektur testikelceller

Article DOI: 10.3791/50819 • 07:34 min • January 10th, 2014
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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Materialet her beskriver en metode udviklet til at bevare den tredimensionelle chromatin struktur testikel kimceller. Dette er blevet kaldt den tredimensionale (3D) diasmetode. Denne metode forbedrer følsomheden for påvisning af subnukleare strukturer og gælder for immunfluorescens, DNA og RNA-fluorescens in situ-hybridisering (FISH).

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Grundlæggende protokol Problem 83 Chromatin Kimceller Kønskromosomer Testis Meiotisk kønskromosominaktivering Postmeiotisk sexkromatin
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