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JoVE Journal Biology
Metodo di preparazione delle diapositive per preservare l'architettura tridimensionale della cromatina delle cellule germinali testicolari
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Metodo di preparazione delle diapositive per preservare l'architettura tridimensionale della cromatina delle cellule germinali testicolari

Article DOI: 10.3791/50819
January 10th, 2014 •
1,2
1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute, Cincinnati Children's Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine

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January 10th, 2014

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Il materiale qui descrive un metodo sviluppato per preservare la struttura tridimensionale della cromatina delle cellule germinali testicolari. Questo è stato definito il metodo di scorrimento tridimensionale (3D). Questo metodo migliora la sensibilità per il rilevamento di strutture subnucleari ed è applicabile per l'immunofluorescenza, il DNA e la fluorescenza dell'RNA nell'ibridazione in situ (FISH).

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