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JoVE Journal Bioengineering
Teste de suscetibilidade de antibiótico induzido por estresse em um chip
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Teste de suscetibilidade de antibiótico induzido por estresse em um chip

Article DOI: 10.3791/50828-v • 12:41 min • January 8th, 2014
January 8th, 2014 •
1, 1, 2, 1,3, 1,4
1Fraunhofer USA Center for Manufacturing Innovation, 2Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 3Department of Mechanical Engineering, Boston University, 4Department of Biomedical Engineering, Boston University

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Desenvolvemos uma plataforma microfluidica para testes rápidos de suscetibilidade a antibióticos. O fluido é passado em altas velocidades sobre bactérias imobilizadas na parte inferior de um canal microfluido. Na presença de estresse e antibiótico, cepas suscetíveis de bactérias morrem rapidamente. No entanto, bactérias resistentes podem sobreviver a essas condições estressantes.

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Bioengenharia Questão 83 antibiótico suscetibilidade resistência microfluidos microscopia rápido testes estresse bactérias fluorescência
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