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Immunology and Infection

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JoVE Journal Immunology and Infection
Etablering af minimal baktericidkoncentration af et antimikrobielt middel til planktoniske celler (MBC-P) og biofilmceller (MBC-B)
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Etablering af minimal baktericidkoncentration af et antimikrobielt middel til planktoniske celler (MBC-P) og biofilmceller (MBC-B)

Article DOI: 10.3791/50854-v • 06:36 min • January 2nd, 2014
January 2nd, 2014 •
1
1Department of Biochemistry, Microbiology and Immunology, University of Ottawa

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Denne protokol giver mulighed for en direkte sammenligning mellem planktonisk og biofilmresistens for en bakteriestamme, der kan danne en biofilm in vitro ved hjælp af en 96-brønds mikrotiterplade. Planktoniske eller biofilmbakterier udsættes for serielle fortyndinger af det valgte antimikrobielle middel. Levedygtigheden dæmpes af vækst på agarplader.

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Immunologi Problem 83 biofilm planktonisk antibiotikaresistens statisk antibakteriel minimal hæmmende koncentration (MIC)
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