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Using Coculture to Detect Chemically Mediated Interspecies Interactions

Article DOI: 10.3791/50863-v • 08:29 min • October 31st, 2013
October 31st, 2013 •
1
1Department of Biology, University of North Carolina at Chapel Hill

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Summary
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Bacteria produce secreted compounds that have the potential to affect the physiology of their microbial neighbors. Here we describe a coculture screen that allows detection of such chemically mediated interspecies interactions by mixing soil microbes with fluorescent transcriptional reporter strains of Bacillus subtilis on solid media.

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Coculture Chemically Mediated Interactions Bacteria Microorganisms Metabolites Fluorescence-based Screen Transcriptional Reporter Strain Solid Media Colonies Biofilm Formation Sporulation Virulence Factor Production Growth Conditions Inducing Metabolite-producing Microbe
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