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Biology

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In vitro Investigação do MexAB efluxo Bomba De Pseudomonas aeruginosa
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In vitro Investigação do MexAB efluxo Bomba De Pseudomonas aeruginosa

Article DOI: 10.3791/50894-v • 13:40 min • February 17th, 2014
February 17th, 2014 •
1, 1, 1, 1
1Laboratoire de Cristallographie & RMN Biologiques, CNRS & Université Paris Descartes

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Nós apresentamos um protocolo para a investigação in vitro de bombas de efluxo de Pseudomonas aeruginosa. Este protocolo permite a geração de um gradiente de protões robusto, reversível, e ajustável dentro da membrana do lipossoma e, portanto, deve ser adaptável a qualquer proteína da membrana energizado pela força protomotive.

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Bioquímica Edição 84 proteína de membrana os transportes a resistência aos antibióticos lipossomas gradiente de prótons bacteriorodopsina
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