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In vitro Undersøgelse af MexAB efflukspumpen Fra Pseudomonas aeruginosa
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In vitro Undersøgelse af MexAB efflukspumpen Fra Pseudomonas aeruginosa

Article DOI: 10.3791/50894-v • 13:40 min • February 17th, 2014
February 17th, 2014 •
1, 1, 1, 1
1Laboratoire de Cristallographie & RMN Biologiques, CNRS & Université Paris Descartes

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Vi præsenterer en protokol for in vitro-undersøgelse af effluxpumper fra Pseudomonas aeruginosa. Denne protokol giver mulighed for generering af en robust, reversible og afstemmelige protongradient i liposommembranen og derfor bør kunne tilpasses enhver membranprotein energi ved protomotive kraft.

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Biochemistry membranprotein transport antibiotikaresistens liposomer protongradient bakterierhodopsin
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