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JoVE Journal Neuroscience
In vivo Fødselsdepression Elektroporation og Time-lapse Imaging af neuroblast Migration i Mouse Akut hjerneskiver
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In vivo Fødselsdepression Elektroporation og Time-lapse Imaging af neuroblast Migration i Mouse Akut hjerneskiver

Article DOI: 10.3791/50905-v • 10:51 min • November 25th, 2013
November 25th, 2013 •
*1, *1, 2, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
* These authors contributed equally

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Neuroblast migration er en grundlæggende begivenhed i postnatal neurogenesis. Vi beskriver en protokol til effektiv mærkning af neuroblasts ved in vivo postnatal elektroporation og efterfølgende visualisering af deres migration ved hjælp af time-lapse billeddannelse af akutte hjernen skiver. Vi inkluderer en beskrivelse af den kvantitative analyse af neuroblast dynamik ved video tracking.

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Neuroscience tidsforskudt Imaging cellevandring Analyser elektroporation neurogenese neuroblast migration neurale stamceller subventricular zone (SVZ) rostrale vandrende stream (RMS) neonatal museunger elektroporation time-lapse imaging hjerne skive kultur sporing celle
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