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JoVE Journal
Chemistry

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A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
 

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Article DOI: 10.3791/50908-v 10:30 min December 27th, 2013
December 27th, 2013

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Summary

The botulinum neurotoxin type A light chain (BoNT/A LC) is a metalloprotease that enters motor neurons, cleaves its substrate SNAP-25, and disrupts neurotransmission, thereby resulting in flaccid paralysis. Utilizing a high-throughput-compatible FRET-based assay, large libraries of small molecules can be screened for their impact on BoNT/A LC enzymatic activity.

Tags

FRET-based Platform Botulinum Neurotoxin Light Chain Modulators High-throughput-compatible Identification Characterization Heavy Chain Internalization Cleaves Intracellular Proteins Neurotransmitter Release Therapies Toxin Binding/internalization Intracellular LC Activity Small Molecules Cellular Permeability Lead Identification High-throughput Screening (HTS) Commercial BoNT/A LC Substrate Recombinant LC Automated HTS Potential BoNT Inhibitors
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