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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitativ bedömning av Human neutrofilmigrationen över en Odlade blåsans epitel
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Kvantitativ bedömning av Human neutrofilmigrationen över en Odlade blåsans epitel

Article DOI: 10.3791/50919-v • 11:20 min • November 7th, 2013
November 7th, 2013 •
1, 1,2
1Department of Pediatrics, Washington University School of Medicine, 2Department of Molecular Microbiology, Washington University School of Medicine

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Vi utvecklade en in vitro-modell som efterliknar en viktig komponent i den akuta inflammatoriska svaret vid infektion i urinblåsan med uropatogena Escherichia coli. Den transuroepithelial neutrofil migration assay möjliggör kvantitativ bedömning av human neutrofil migration över blåsan epitel, odlades på permeabla bärare, som svar på bakterieinfektion eller kemo-attraherande ämnen.

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Immunologi uropatogena Neutrofiler blåsans epitel neutrofil migration medfödd immunitet urinvägsinfektion
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