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Journal / Bioengineering / Patterning cellulare su fotolitograficamente…
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Bioengineering

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JoVE Journal Bioengineering
Patterning cellulare su fotolitograficamente Definito parylene-C
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Patterning cellulare su fotolitograficamente Definito parylene-C: SiO

Article DOI: 10.3791/50929 • 07:19 min
March 7th, 2014 •
1, 2, 3, 1, 3
1Centre for Integrative Physiology, School of Biomedical Sciences, The University of Edinburgh, 2Edinburgh Cancer Research Centre, Institute of Genetics and Molecular Medicine, Western General Hospital, 3School of Engineering, Institute for Integrated Micro and Nano Systems, The University of Edinburgh

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March 7th, 2014

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Questo protocollo descrive un metodo compatibile microfabbricazione per patterning cellulare su SiO 2. Un disegno predefinito parylene-C è fotolitograficamente stampato su SiO 2 wafer. Dopo l'incubazione con siero (o altra soluzione di attivazione) cellule aderiscono specificamente (e crescere secondo la conformità dei) sottostante parylene-C, pur essendo repulsione per SiO 2 regioni.

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