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Journal / Biology / Genoma Ingegneria mouse usando Nucleases Designer
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Genoma Ingegneria mouse usando Nucleases Designer

Article DOI: 10.3791/50930-v • 12:04 min • April 2nd, 2014
April 2nd, 2014 •
1, 2, 2, 1
1Institute of Laboratory Animal Science, University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering, University of Minnesota

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Nucleasi di stilisti come nucleasi dito di zinco (ZFNs) e attivatore di trascrizione come nucleases effettrici (Talens) possono essere utilizzati per modificare il genoma di embrioni preimpianto di topo attivando sia la fine nonhomologous giunzione (NHEJ) e la ricombinazione omologa (HR) percorsi. Questi progressi consentono la rapida generazione di mouse con precise modificazioni genetiche.

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Genetica microiniezione degli ovociti nucleasi Designer ZFN TALEN Genome Ingegneria
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