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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering av myeloid dendrittiske celler og Epitelceller fra Menneskelig Thymus
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Isolering av myeloid dendrittiske celler og Epitelceller fra Menneskelig Thymus

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Denne protokollen detaljer en metode for å isolere antigen-presenterende celler fra human thymus via ulike trinn av enzymatisk nedbrytning av vevet etterfulgt av tetthetssentrifugering av enkeltcellesuspensjonen og til slutt magnetisk og / eller FACS sortering av cellepopulasjoner av interesse.

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Immunologi immunsystemprosesser biologiske prosesser immunologi immunsystem sykdommer immunsystem Phenomena miljø-og biovitenskap (General) immunologi menneskelig thymus isolasjon dendrittiske celler Mtec CTEC
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