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O isolamento de células dendríticas mielóides e células epiteliais de timo humano

Article DOI: 10.3791/50951-v • 09:07 min • September 19th, 2013
September 19th, 2013 •
1,2, 1, 3, 4, 5, 1
1Department of General Neurology, Hertie Institute for Clinical Brain Research, 2Institute of Pharmacology, University of Bern, 3Department of Immunology, University Medical Center Hamburg-Eppendorf, 4Department of Thoracic and Cardiovascular Surgery, University Clinic Tuebingen, 5Department of Neurology, University Hospital Erlangen

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Este protocolo detalha um método para isolar células apresentadoras de antigénio a partir de timo humano por meio de diferentes passos de digestão enzimática do tecido seguida por centrifugação de densidade da suspensão de células individuais e finalmente separação magnética e / ou FACS das populações de células de interesse.

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Imunologia Edição 79 Processos do Sistema Imunológico processos biológicos imunologia Doenças do Sistema Imune Fenômenos do Sistema Imunológico Ciências Biológicas (Geral) imunologia timo humano o isolamento as células dendríticas Mtec CTEC
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