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Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
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Chapters
- 00:05Title
- 01:38Preparation of L. pneumophila for Infection
- 03:04Preparation and Infection of G. mellonella
- 05:39Analysis of Larval Mortality
- 06:17Extraction and Processing of Hemolymph
- 08:57Quantifying the Bacterial CFU
- 09:37Virulence of WT and Dot/Icm-deficient L. pneumophila
- 12:07Conclusion
The larva of the wax moth Galleria mellonella was recently established as an in vivo model to study Legionella pneumophila infection. Here, we demonstrate fundamental techniques to characterize the pathogenesis of Legionella in the larvae, including inoculation, measurement of bacterial virulence and replication as well as extraction and analysis of infected hemocytes.
