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JoVE Journal
Biology

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Detection of Live Escherichia coli O157
 

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

Article DOI: 10.3791/50967
February 1st, 2014

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Summary February 1st, 2014

A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples

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