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JoVE Journal Biology
Påvisning av Levende
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
Journal (Biology)
Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
Journal (Biology)
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
 
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Påvisning av Levende

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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En qPCR analysen ble utviklet for påvisning av Escherichia coli O157: H7 målretting en unik genetisk markør, Z3276. QPCR ble kombinert med propidium monoazide (PMA) behandling for levende celle gjenkjenning. Denne protokoll er blitt modifisert og tilpasset til en 96-brønn plate-format for enkel og jevn behandling av mange prøver

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Mikrobiologi Propidium monoazide (PMA) real-time PCR, patogen selektiv deteksjon levende celler
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