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Journal / Biology / Detection Live
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Biology

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JoVE Journal Biology
Detection Live
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
Journal (Biology)
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate
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Detection Live

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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En qPCR assay blev udviklet til påvisning af Escherichia coli O157: H7 rettet mod en unik genetisk markør, Z3276. QPCR blev kombineret med propidium monoazide (PMA) behandling til påvisning levende celle. Denne protokol er blevet modificeret og tilpasset til en 96-brønds plade format for nem og ensartet håndtering af talrige prøver

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Mikrobiologi Propidium monoazide (PMA) real-time PCR, patogen selektiv detektion levende celler
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