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Biology

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JoVE Journal Biology
Detecção de Vivo
Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
Journal (Biology)
Concentration of Metabolites from Low-density Planktonic Communities for Environmental Metabolomics using Nuclear Magnetic Resonance Spectroscopy
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
Journal (Biology)
Culturing, Freezing, Processing, and Imaging of Entire Organoids and Spheroids While Still in a Hydrogel
 
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Detecção de Vivo

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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Um ensaio de qPCR foi desenvolvido para a detecção de Escherichia coli O157: H7 alvo um marcador genético único, Z3276. A qPCR foi combinado com propidium monoazide (PMA) o tratamento para a detecção de células vivas. Este protocolo foi modificado e adaptado a um formato de placa de 96 poços para uma fácil manipulação e consistente de numerosas amostras

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Microbiologia Edição 84 propídio monoazide (PMA) PCR em tempo real, patógenos detecção seletiva as células vivas
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