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Biology

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JoVE Journal Biology
Upptäckt av Live
Author Spotlight: The Production of Recombinant Proteins (Video) | JoVE
Journal (Biology)
Author Spotlight: The Production of Recombinant Proteins (Video) | JoVE
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Journal (Biology)
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
 
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Upptäckt av Live

Article DOI: 10.3791/50967-v • 08:16 min • February 1st, 2014
February 1st, 2014 •
1, 1, 1
1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

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En qPCR analys utvecklades för detektion av Escherichia coli O157: H7 riktar en unik genetisk markör, Z3276. Den qPCR kombinerades med propidium monoazide (PMA) behandling för levande cell upptäckt. Detta protokoll har ändrats och anpassats till en 96-brunnar format för enkel och konsekvent hantering av många prover

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Mikrobiologi Propidium monoazide (PMA) realtids-PCR, patogen selektiv upptäckt levande celler
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