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JoVE Journal Chemistry
Submillisecond konformationsændringer i Proteiner løst ved Photothermal Beam Nedbøjning
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Submillisecond konformationsændringer i Proteiner løst ved Photothermal Beam Nedbøjning

Article DOI: 10.3791/50969-v • 10:02 min • February 18th, 2014
February 18th, 2014 •
1, 1
1Department of Chemistry and Biochemistry, Florida International University

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Her rapporterer vi en anvendelse af photothermal stråle afbøjning teknik i kombination med et bur calciumkomponent, DM-nitrophen, overvåge mikrosekund og millisekund dynamik og energetik af strukturelle ændringer i forbindelse med calcium association til en neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator .

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Kemi photothermal teknikker photothermal stråle deformation volumen forandring enthalpiændring calcium sensorer kalium kanal interaktion protein DM-nitrophen
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