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Kültür Hücrelerinin retikulum Subdomain'ler Görselleştirme
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Kültür Hücrelerinin retikulum Subdomain'ler Görselleştirme

Article DOI: 10.3791/50985-v • 16:43 min • February 18th, 2014
February 18th, 2014 •
1,2,3, 2,3,4, 2,3, 1,2
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine, University of Milan, 3Neuroscience Institute, National Research Council (CNR), 4Department of Health Science, "Magna Graecia" University of Catanzaro

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Biz canlı hücre konfokal görüntüleme vasıtasıyla, endoplazmik retikulum (ER) içerisinde yerleşik transfekte floresan protein dağılımı ve hareketliliği incelemek için kullanılan görüntüleme yaklaşımı tarif eder. Biz de ultrastruktural bu subselüler bölmesinin mimarisi üzerine kendi ifade etkisini analiz.

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Mikrobiyoloji Sayı 84 Endoplazmik retikulum (ER) floresan proteinleri (FP) konfokal mikroskopi photobleaching sonra floresan kurtarma (sıkı bağlamak) ışıkla floresan kaybı (FLIP) ince transmisyon elektron mikroskobu (TEM)
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