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Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
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Neuroscience
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JoVE Journal Neuroscience
Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

DOI:
10.3791/50987-v

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10:55 min

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May 03, 2014

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, , ,
1Laboratory of Neurodevelopement and Repair,University of Science and Technology of China

Chapters

  • 00:00Title
  • 00:55Introduction
  • 01:17Construct the apparatus
  • 01:47Prepare reagents and tools
  • 03:20Begin the surgery
  • 07:26Wholemounting retina and imaging
  • 10:30Representative results

Summary

Automatic Translation

Automatic Translation

We introduce an efficient method to retrograde label retinal ganglion cells (RGCs) in adult zebrafish.

Tags

Retrograde LabelingRetinal Ganglion CellsRGCsFluorescent DyesAdult ZebrafishOptic Nerve InjuryRGCs SurvivalRGCs RegenerationAcid-etchingLight Curing BondOptic Nerve Crush

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