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Journal / Neuroscience / Nucleofection av Rodent neuroblasts å studere…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection av Rodent neuroblasts å studere neuroblast Migration
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Nucleofection av Rodent neuroblasts å studere neuroblast Migration

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Neuroblast migrasjon er et viktig skritt i postnatal neurogenesis. Protokollen er beskrevet her kan brukes til å undersøke hvilken rolle kandidat regulatorer av neuroblast migrasjon ved å bruke DNA / liten hårnål RNA (shRNA) nucleofection og en 3D migrasjon analysen med neuroblasts isolert fra gnager postnatal rostral migrasjonsstrømmen.

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Neuroscience cellebiologi cellemigrasjon Analyser Transfection Neurogenesis subventricular sone (SVZ) nevrale stamceller rostral migrasjonsstrømmen (RMS) neuroblast 3D migrasjon analysen nucleofection
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