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JoVE Journal
Neuroscience

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Nucleofection gnaver neuroblasts til Studieinformationen neuroblast Migration
 
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Nucleofection gnaver neuroblasts til Studieinformationen neuroblast Migration

Article DOI: 10.3791/50989-v 11:32 min November 12th, 2013
November 12th, 2013

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Summary

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Click here for the English version.

Neuroblast migration er et afgørende skridt i postnatal neurogenesis. Protokollen beskrevet her kan bruges til at undersøge, hvilken rolle af kandidat regulatorer af neuroblast migration ved at ansætte DNA / lille hårnål RNA (shRNA) nucleofection og en 3D migration assay med neuroblasts isoleret fra gnaver postnatal rostral vandrende stream.

Tags

Neuroscience cellebiologi cellemigration Analyser transfektion Neurogenese subventricular zone (SVZ) neurale stamceller rostrale vandrende stream (RMS) neuroblast 3D migration assay nucleofection
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