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Journal / Neuroscience / Nucleofection von Nagerneuroblasten zu Neuroblast …
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection von Nagerneuroblasten zu Neuroblast Migrationsstudie
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Nucleofection von Nagerneuroblasten zu Neuroblast Migrationsstudie

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Neuroblast Migration ist ein entscheidender Schritt in der postnatalen Neurogenese. Das hier beschriebene Protokoll kann verwendet werden, um die Rolle der Regulatoren der Neuroblast Kandidat Migration durch den Einsatz von DNA / kleine Haarnadel-RNA (shRNA) Nukleofektion und eine 3D-Migrationsassay mit Neuroblasten aus dem Nagetier postnatale rostralen Migrationsstrom getrennt zu untersuchen.

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Neuroscience Ausgabe 81 Zellbiologie Zellmigrationsassays Transfektion Neurogenese Subventrikularzone (SVZ) neurale Stammzellen rostralen Migrationsstrom (RMS) Neuroblast 3D-Migrationsassay Nukleofektion
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