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Journal / Neuroscience / Nucleofection av Gnagare neuroblaster att studera …
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection av Gnagare neuroblaster att studera neuroblast migration
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Nucleofection av Gnagare neuroblaster att studera neuroblast migration

Article DOI: 10.3791/50989-v • 11:32 min • November 12th, 2013
November 12th, 2013 •
*1, *2, 1, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2MRC Centre for Developmental Neurobiology, King's College London
* These authors contributed equally

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Neuroblast migration är ett avgörande steg i postnatal neurogenes. Protokollet som beskrivs här kan användas för att undersöka rollen av kandidat regulatorer av neuroblast migration genom användning av DNA / liten hårnål RNA (shRNA) nucleofection och en 3D-migration assay med neuroblaster som isolerats från gnagare postnatal rostral migratory stream.

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Neurovetenskap Cellular Biology Cell Migration Analyser transfektion neurogenes subventrikulära zonen (SVZ) neurala stamceller rostralt vandrande ström (RMS) neuroblast 3D migrationsanalys nucleofection
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