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Journal / Bioengineering / Att använda Mikrofluidik Chips för Live…
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Bioengineering

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JoVE Journal Bioengineering
Att använda Mikrofluidik Chips för Live avbildning och studier av Skade svar i
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
Journal (Bioengineering)
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition
Journal (Bioengineering)
High Throughput Traction Force Microscopy Using PDMS Reveals Dose-Dependent Effects of Transforming Growth Factor-β on the Epithelial-to-Mesenchymal Transition
 
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Att använda Mikrofluidik Chips för Live avbildning och studier av Skade svar i

Article DOI: 10.3791/50998-v • 11:46 min • February 7th, 2014
February 7th, 2014 •
1, 2, 1, 1, 1, 3,4, 2,5, 1
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Life Sciences Institute, University of Michigan, 4Department of Cell and Developmental Biology, University of Michigan, 5Department of Mechanical Engineering, University of Michigan

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Drosophila larver är ett attraktivt modellsystem för levande avbildning på grund av deras genomskinliga nagelband och kraftfulla genetik. Detta protokoll beskriver hur man använder ett enda lager PDMS enhet, kallad "larv-chip" för live-avbildning av cellulära processer inom nervceller i 3: e INSTAR Drosophila larver.

Tags

Bioteknik , Live avbildning Microfluidics axonal skada axonal degeneration kalcium avbildning photoconversion lasermikrokirurgi
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