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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento de Cortical Microglia com Imunofenótipo preservada e funcionalidades de murino Os recém-nascidos
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Isolamento de Cortical Microglia com Imunofenótipo preservada e funcionalidades de murino Os recém-nascidos

Article DOI: 10.3791/51005-v • 09:12 min • January 30th, 2014
January 30th, 2014 •
1, 1, 1
1Department of Neuroscience, Georgetown University Medical Center

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Uma chave para o sucesso na investigação de biologia da microglia é a preservação do immunofunction microglial ex vivo durante o isolamento a partir de tecido do SNC. Isolando microglia através de resultados de agitação rotativos em culturas de células altamente puras e imuno avaliado pela imagem fluorescente, imunocitoquímica e ELISA após a ativação da microglia com o lipopolissacarídeo estímulos pró-inflamatória (LPS) e Pam 3 CSK 4 (Pam).

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Imunologia Edição 83 neuroinflamação citocinas neurodegeneração LPS Pam3CSK4 TLRs PAMPs DAMPs
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