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JoVE Journal Bioengineering
Um método aperfeiçoado para a preparação de colágeno tipo I Da Pele
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Um método aperfeiçoado para a preparação de colágeno tipo I Da Pele

Article DOI: 10.3791/51011-v • 05:17 min • January 21st, 2014
January 21st, 2014 •
1,2, 1, 1,2
1Department of Anesthesiology, Perioperative and Pain Medicine, Boston Children's Hospital, 2Department of Anesthesia, Harvard Medical School

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Procedimentos tradicionais para o isolamento do tipo solúvel 1 colágeno (col1) requerem cerca de 10 dias do início ao fim por causa de incubações tampão longas e trabalhosas resuspensions de fibrilas. Aqui, nós descrevemos um meio para purificar a partir de pequenas biópsias COL1 dérmicos em menos de 3 horas.

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Bioengenharia colágeno tipo 1 matriz extracelular a engenharia de tecidos a proteína andaime derme cório
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