A subscription to JoVE is required to view this content. Sign in or start your free trial.
22,837 Views
•
08:32 min
•
December 13, 2013
DOI:
Summary
Automatically generated
The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.
Read Article
Cite this Article
Ivanova, E., Toychiev, A. H., Yee, C. W., Sagdullaev, B. T. Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry. J. Vis. Exp. (82), e51018, doi:10.3791/51018 (2013).
Copy