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Görüntüleme ve İmmünhistokimyanın için Retina Wholemount Hazırlık için optimize edilmiş Protokolü
JoVE Journal
Biology
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JoVE Journal Biology
Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry

Görüntüleme ve İmmünhistokimyanın için Retina Wholemount Hazırlık için optimize edilmiş Protokolü

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08:32 min

December 13, 2013

DOI:

08:32 min
December 13, 2013

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Summary

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The protocol described here is for structural assessment of a wholemount retinal preparation. This includes descriptions of tissue dissection, mounting onto a hydrophilized polytetrafluoroethylene (PTFE) membrane insert, bolus loading with fluorescent markers, and a comparison of fixation with carbodiimide and paraformaldehyde for immunohistochemical analysis of cellular and synaptic components.

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