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Journal / Immunology and Infection / Fluoreszenz
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Immunology and Infection

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Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Journal (Immunology and Infection)
Retroviral Overexpression of CXCR4 on Murine B-1a Cells and Adoptive Transfer for Targeted B-1a Cell Migration to the Bone Marrow and IgM Production
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
Journal (Immunology and Infection)
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
 
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Fluoreszenz

Article DOI: 10.3791/51030-v • 10:38 min • February 24th, 2014
February 24th, 2014 •
1,2, 1, 1, 1, 1, 1, 1,3, 4, 2, 1
1Department of Entomology, Volcani Center, 2Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, 3Department of Applied Sciences, Institute for Adriatic Crops and Karst Reclamation, 4The Institute of Plant Sciences, Volcani Center

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Wir beschreiben hier ein einfaches Fluoreszenz in situ Hybridisierung (FISH)-Verfahren zur Lokalisierung von Viren und Bakterien in Insekten-und Pflanzengeweben. Dieses Protokoll kann für die Visualisierung von mRNA in ganze Berg und mikroskopische Abschnitte erweitert werden.

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Infektion FISH Lokalisierung Insekten Pflanzen Viren Endosymbionten Fixierung konfokale Mikroskopie
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