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JoVE Journal
Immunology and Infection

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VIGS介导的正向遗传学筛选参与非寄主抗性基因鉴定
 
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VIGS介导的正向遗传学筛选参与非寄主抗性基因鉴定

Article doi: 10.3791/51033
August 23rd, 2013

Summary August 23rd, 2013

Please note that all translations are automatically generated.

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病毒诱导的基因沉默是一个有用的工具,用于识别植物非寄主抗性基因参与。我们演示了如何使用中表达GFPuv的识别基因沉默植株易受非寄主病原体的细菌病原体。这种做法是方便,快捷,有利于大规模筛选和类似协议可以应用到其他各种植物与微生物相互作用研究。

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