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Immunology and Infection
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Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
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Immunology and Infection
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JoVE Journal Immunology and Infection
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes

DOI:
10.3791/51043-v

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14:05 min

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September 19, 2013

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, , , , , ,
1Unité des Interactions Bactéries Cellules,Pasteur Institute, 2INSERM U604, 3Institut National de la Recherche Agronomique (INRA), USC2020, 4Institute of Biochemistry,ETH Zürich, 5Focal Area Infection Biology, Biozentrum,University of Basel

Chapters

  • 00:05Title
  • 01:39Reverse siRNA Cell Transfection
  • 04:14Cellular Infection
  • 06:57Staining for InlC Protein
  • 09:19Results: Cell Infection by L. monocytogenes is Detected by Fluorescent Labeling of Cytoplasmic InlC
  • 13:27Conclusion

Summary

Automatic Translation

Automatic Translation

Listeria monocytogenes is a Gram positive bacterial pathogen frequently used as a major model for the study of intracellular parasitism. Imaging late L. monocytogenes infection stages within the context of small-interfering RNA screens allows for the global study of cellular pathways required for bacterial infection of target host cells.

Tags

Keywords: Listeria MonocytogenesBacterial PathogenIntracellular ParasitismCellular Immune ResponseInlCFluorescent LabelingMicroscopyHigh-throughput SiRNA ScreeningCellular Signaling Pathways

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