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JoVE Journal
Biology

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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
 
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Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Article DOI: 10.3791/51060-v 08:20 min December 20th, 2013
December 20th, 2013

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Summary

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Click here for the English version.

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

Tags

Apoptosis Analysis Zebrafish Embryos Whole-mount Immunofluorescence Activated Caspase 3 Casp3 Assay Intrinsic Apoptosis Extrinsic Apoptosis Tissue Specificity Cell Types Fixed Embryos 4-cell Stage 32 Hr-post-fertilization Zebrafish Mutants Zebrafish Morphants Overexpression Of Mutant And Wild-type MRNAs Exposure To Chemicals Acridine Orange Staining TUNEL Assay
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