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Journal / Immunology and Infection / 맹장 결찰 및 마우스에서 autophagy에 공부를 모델로 패혈증 관통…
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Immunology and Infection

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JoVE Journal Immunology and Infection
맹장 결찰 및 마우스에서 autophagy에 공부를 모델로 패혈증 관통 유도
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맹장 결찰 및 마우스에서 autophagy에 공부를 모델로 패혈증 관통 유도

Article DOI: 10.3791/51066-v • 06:40 min • February 9th, 2014
February 9th, 2014 •
1,3, 1, 2, 2, 1, 1
1Department of Medicine, Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, 2Department of Medicine, Renal Division, Brigham and Women's Hospital, Harvard Medical School, 3First Department of Critical Care Medicine and Pulmonary Services, University of Athens Medical School, Evangelismos Hospital, Athens, Greece

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실험 패혈증은 맹장 결찰과 천자 (CLP) 방법을 사용하여 마우스에서 유발 될 수있다. CLP - 유도 패혈증의 컨텍스트에서 생체에 autophagy를 평가하는 현재의 프로토콜이 여기에 제시된다 : (GFP) - LC3 마우스를 사용 autophagy에 측정을위한 프로토콜, 및 전자 현미경에 의해 autophagosome 형성을 측정하기위한 프로토콜.

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감염 제 84 autophagosome autophagy에 맹장 결찰술과 펑크 마우스 패혈증
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